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Effect of medium pH on shoot regeneration from the cotyledonary explants of tomato

journal contribution
posted on 06.12.2017, 00:00 authored by Poonam BhatiaPoonam Bhatia, Nanjappa AshwathNanjappa Ashwath
To optimise medium pH for in vitro shoot regeneration and shoot growth from the cotyledon explants of tomato cv. Red Coat, the explants were innoculated onto MS medium which was maintained at a wide range of pH (4.5-7.5). Percentage shoot regeneration and number of shoots produced per explant were not significantly affected by the medium pH. The proportion of explants that produced callus and callus diameter remained unaffected by the medium pH. In contrast, the shoot height was significantly affected and taller shoots were only produced at a pH range of 5.5-6.0. Lower (4.5-5) and higher (6.5-7.5) medium pH significantly reduced shoot height. The results suggest that the explants of tomato Cv. Red Coat are moderately tolerant to a wide range of medium pH, as most of the traits studied remained unaffected by the change in media pH. However, the best regeneration and growth occurred only in the pH range of 5.5-6.0.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

4

Issue

1

Start Page

7

End Page

10

Number of Pages

4

ISSN

1682-296X

Location

Pakistan

Publisher

Asian Network for Scientific Information

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Faculty of Arts, Health and Sciences; TBA Research Institute;

Era Eligible

Yes

Journal

Biotechnology.