File(s) not publicly available

Detectable effect size and bioassay power of mummichog (Fundulus heteroclitus) and fathead minnow (Pimephales promelas) adult reproductive tests

journal contribution
posted on 06.12.2017, 00:00 authored by Steven MelvinSteven Melvin, K Munkittrick, T Bosker, D MacLatchy
Although multiple reproductive tests have been developed in small-bodied fish to determine the effects of endocrine-disrupting substances, few direct comparisons have been made among the available tests. Side-by-side reproductive tests with mummichog (Fundulus heteroclitus) and fathead minnow (FHM; Pimephales promelas) were conducted with 0, 3, 10, and 30% effluent from a bleached kraft pulp mill in Saint John, New Brunswick, Canada. Egg production was significantly increased in mummichog exposed to 3% combined mill effluent, but no difference was observed in FHM. No differences were found in whole-body testosterone or estradiol levels in mummichog, and whole-body 11-ketotestosterone levels in males were increased in 3% exposed fish compared to those in 10% effluent. Male FHM exposed to 30% effluent had increased whole-body testosterone levels, and female FHM in 30% effluent had decreased testosterone. No differences in estradiol or11-ketotestosterone were observed in FHM. Relatively limited response occurred in other endpoints. A comparison of the results of the present study to other published studies suggests that current reproductive bioassays are only sensitive for detecting magnitudes of change of greater than 50% and that differences exist in the sensitivities of fish. Future research should address methods of reducing variability within test populations and focus on understanding the comparative responses among species commonly used for endocrine-disrupting substance testing.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

28

Issue

11

Start Page

2416

End Page

2425

Number of Pages

10

eISSN

1552-8618

ISSN

0730-7268

Location

USA

Publisher

Jossey Bass

Language

en-aus

Peer Reviewed

Yes

Open Access

No

Era Eligible

Yes

Journal

Environmental toxicology and chemistry.