CQUniversity
Browse
1/1
2 files

Culture and stimulation of Tammar Wallaby Lymphocytes

journal contribution
posted on 2017-12-06, 00:00 authored by Lauren Young, E Deane
We describe the culture and stimulation of lymphocytes from the model marsupial, the Tammar Wallaby (Macropus eugenii). We also describe the capacity of Tammar Wallaby lymphocytes isolated from blood, spleen and lymph nodes to produce soluble immunomodulatory factors. Culture conditions were optimized for mitogen-driven stimulation using the plant lectin phytohaemagglutinin (PHA). Products secreted by stimulated cells were harvested and crudely fractionated before they were added back to freshly isolated lymphocytes. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay, both stimulatory and inhibitory bioactive factors were detected in serum-free supernatants harvested from mitogen-treated peripheral blood mononuclear cells. This paper describes the capacity of leukocytes of the tammar wallaby to respond to mitogenic stimulation and to produce soluble, low-molecular-weight bioactive molecules that possess cytokine-like activity.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

31

Issue

6

Start Page

685

End Page

701

Number of Pages

17

eISSN

1573-7446

ISSN

0165-7380

Location

Netherlands

Publisher

Springer

Language

en-aus

Peer Reviewed

  • Yes

Open Access

  • No

External Author Affiliations

Division of Environmental and Life Sciences; Faculty of Sciences, Engineering and Health;

Era Eligible

  • Yes

Journal

Veterinary research communications.

Usage metrics

    CQUniversity

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC