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Correlations between intestinal innate immune genes and cecal microbiota highlight potential for probiotic development for immune modulation in poultry

journal contribution
posted on 31.05.2019, 00:00 by N-L Willson, GS Nattrass, RJ Hughes, RJ Moore, Dragana StanleyDragana Stanley, PI Hynd, REA Forder
Immune function is influenced by the diversity and stability of the intestinal microbiota. A likely trade-off of immune function for growth has been demonstrated in heavier breeds of poultry that have been genetically selected for growth and feed efficiency traits. We investigated the expression of selected innate immune genes and genes encoding products involved in intestinal barrier function to determine whether function changes could be consistently linked to the phenotypic expression of feed conversion ratio (FCR), a common measure of performance within poultry broiler flocks. In addition, we compared individual cecal microbial composition with innate immune gene expression. Samples were utilised from two replicate trials termed P1E1 and P1E2. High (n = 12) and low (n = 12) performing birds were selected based on their individual FCR data from each replicate and combined for microbiota phylogenetic composition and immune gene expression analysis. Toll-like receptor 1 (TLR1La) and zonula occludens 1 (ZO1) were differentially expressed between high- and low-performing broilers. Several taxa were correlated with FCR; of these, unclassified YS2 and ZO1 were also positively correlated with each other. Interactions between taxa and differentially expressed innate immune genes between P1E1 and P1E2 were much greater compared to relationships between high- and low-performing birds. At the level of phylum, reciprocal correlations between tight junction proteins and Toll-like receptors with Bacteroidetes and Firmicutes were evident, as were correlations at the genus level. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Funding

Category 3 - Industry and Other Research Income

History

Volume

102

Issue

21

Start Page

9317

End Page

9329

Number of Pages

13

eISSN

1432-0614

ISSN

0175-7598

Publisher

Springer, Germany

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

University of Adelaide; University of New England; RMIT University; South Australian Research and Development Institute (SARDI)

Author Research Institute

Institute for Future Farming Systems

Era Eligible

Yes

Journal

Applied Microbiology and Biotechnology