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Challenging immunodominance of influenza-specific CD8+ T cell responses restricted by the risk-associated HLA-A*68:01 allomorph

journal contribution
posted on 30.04.2020, 00:00 by CE van de Sandt, EB Clemens, EJ Grant, LC Rowntree, S Sant, H Halim, J Crowe, AC Cheng, TC Kotsimbos, M Richards
Although influenza viruses lead to severe illness in high-risk populations, host genetic factors associated with severe disease are largely unknown. As the HLA-A*68:01 allele can be linked to severe pandemic 2009-H1N1 disease, we investigate a potential impairment of HLA-A*68:01-restricted CD8+ T cells to mount robust responses. We elucidate the HLA-A*68:01+CD8+ T cell response directed toward an extended influenza-derived nucleoprotein (NP) peptide and show that only ~35% individuals have immunodominant A68/NP145+CD8+ T cell responses. Dissecting A68/NP145+CD8+ T cells in low vs. medium/high responders reveals that high responding donors have A68/NP145+CD8+ memory T cells with clonally expanded TCRαβs, while low-responders display A68/NP145+CD8+ T cells with predominantly naïve phenotypes and non-expanded TCRαβs. Single-cell index sorting and TCRαβ analyses link expansion of A68/NP145+CD8+ T cells to their memory potential. Our study demonstrates the immunodominance potential of influenza-specific CD8+ T cells presented by a risk HLA-A*68:01 molecule and advocates for priming CD8+ T cell compartments in HLA-A*68:01-expressing individuals for establishment of pre-existing protective memory T cell pools. © 2019, Crown.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

10

Start Page

1

End Page

16

Number of Pages

16

eISSN

2041-1723

Publisher

Nature Publishing Group

Additional Rights

CC BY 4.0

Peer Reviewed

Yes

Open Access

Yes

External Author Affiliations

La Trobe University; Griffith University; The Alfred Hospital, Melbourne; University of Melbourne at The Peter Doherty Institute; Monash University; 4Deepdene Surgery, Vic.

Era Eligible

Yes

Journal

Nature Communications