The study scrutinised the impact of the adoption of Global Reporting Initiative's (GRI) G4 guidelines on Australian listed companies’ biodiversity reporting and examined the change of biodiversity reporting from the pre-G4 to the post-G4 era. The impacts of certain firm-specific factors on biodiversity reporting were also examined. Our results show a significant increase in biodiversity reporting during the G4 era as compared to the G3 era, indicating an improved voluntary reporting regime facilitates the extent of biodiversity reporting and introduces more transparency and accountability. The use of GRI’s G4 guidelines, greater media exposure, metals and mining industry membership of companies, and smaller board size increases the extent of firms’ biodiversity disclosure but firm size and profitability do not affect the extent of biodiversity disclosure. Australian biodiversity disclosure increased significantly over time …