File(s) not publicly available

Bi-functional amperometric biosensor for low concentration hydrogen peroxide measurements using polypyrrole immobilizing matrix

journal contribution
posted on 06.12.2017, 00:00 by E Mala Ekanayake, Daluwathu PreethichandraDaluwathu Preethichandra, K Kaneto
Usage of nanoporous polypyrrole on H²O² bi-functional sensing after immobilizing horseradish peroxidase (HRP) is described in this paper. Nanoporous alumina membranes were used to produce nanopores on polypyrrole surface. Nanopores increased the effective surface area available for enzyme immobilizing. Further, this increased area enhances the electron flow caused by the catalytic reactions. When the sensor was deployed to sense H²O² at 0.2V a high sensitivity of 3.9AM¯¹ cm¯² was observed at a fast response time of 5 s. When the working potential was reversed to −0.1V, a wider linear range of 10nM to 25 µM was achieved. These novel findings will lead the way to a new path of biosensor design for low substrate concentrations with enhanced characteristics.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

132

Start Page

166

End Page

171

Number of Pages

6

eISSN

1873-3077

ISSN

0925-4005

Location

Switzerland

Publisher

Elsevier S.A.

Language

en-aus

Peer Reviewed

Yes

Open Access

No

Era Eligible

Yes

Journal

Sensors and actuators. B, Chemical.