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Bi-functional amperometric biosensor for low concentration hydrogen peroxide measurements using polypyrrole immobilizing matrix
journal contribution
posted on 2017-12-06, 00:00 authored by E Mala Ekanayake, Daluwathu PreethichandraDaluwathu Preethichandra, K KanetoUsage of nanoporous polypyrrole on H²O² bi-functional sensing after immobilizing horseradish peroxidase (HRP) is described in this paper. Nanoporous alumina membranes were used to produce nanopores on polypyrrole surface. Nanopores increased the effective surface area available for enzyme immobilizing. Further, this increased area enhances the electron flow caused by the catalytic reactions. When the sensor was deployed to sense H²O² at 0.2V a high sensitivity of 3.9AM¯¹ cm¯² was observed at a fast response time of 5 s. When the working potential was reversed to −0.1V, a wider linear range of 10nM to 25 µM was achieved. These novel findings will lead the way to a new path of biosensor design for low substrate concentrations with enhanced characteristics.
Funding
Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)
History
Volume
132Start Page
166End Page
171Number of Pages
6eISSN
1873-3077ISSN
0925-4005Location
SwitzerlandPublisher
Elsevier S.A.Publisher DOI
Full Text URL
Language
en-ausPeer Reviewed
- Yes
Open Access
- No
Era Eligible
- Yes