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Antifibrotic activity of an inhibitor of histone deacetylases in DOCA-salt hypertensive rats

journal contribution
posted on 06.12.2017, 00:00 authored by A Iyer, Andrew FenningAndrew Fenning, J Lim, G Le, R Reid, M Halili, D Fairlie, L Brown
Background and purpose: Histone deacetylases (HDACs) silence genes by deacetylating lysine residues in histones and other proteins. HDAC inhibitors represent a new class of compounds with anti-inflammatory activity. This study investigated whether treatment with a broad spectrum HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), would prevent cardiac fibrosis, part of the cardiovascular remodelling in deoxycorticosterone acetate (DOCA)-salt rats. Experimental approach: Control and DOCA-salt rats were treated with SAHA (25 mg·kg−1·day−1 s.c.) for 32 days. Changes in cardiovascular structure and function were assessed by blood pressure in vivo and in Langendorff perfused hearts, ventricular papillary muscle and in aortic rings in vitro. Left ventricular collagen deposition was assessed by histology. Key results: Administration of SAHA to DOCA-salt rats attenuated the following parameters: the increased concentration of over 20 pro-inflammatory cytokines in plasma, increased inflammatory cell infiltration and interstitial collagen deposition, increased passive diastolic stiffness in perfused hearts, prolongation of action potential duration at 20% and 90% of repolarization in papillary muscle, development of left ventricular hypertrophy, systolic hypertension and changes in vascular dysfunction. Conclusions and implications: The HDAC inhibitor, SAHA, attenuated the cardiovascular remodelling associated with DOCA-salt hypertensive rats and improved cardiovascular structure and function, especially fibrosis, in the heart and blood vessels, possibly by suppressing inflammation. Control of cardiac histone or non-histone protein acetylation is a potential therapeutic approach to preventing cardiac remodelling, especially cardiac fibrosis.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

159

Issue

7

Start Page

1408

End Page

1417

Number of Pages

10

ISSN

0007-1188

Location

UK

Publisher

John Wiley

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Faculty of Sciences, Engineering and Health; Institute for Health and Social Science Research (IHSSR); Institute of Molecular Biosciences; School of Biomedical Sciences;

Era Eligible

Yes

Journal

British journal of pharmacology.