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An amperometric glucose biosensor with enhanced measurement stability and sensitivity using an artificially porous conducting polymer

journal contribution
posted on 06.12.2017, 00:00 by E Mala Ekanayake, Daluwathu Preethichandra, K Kaneto
A conducting polymer [polypyrrole (PPy)]-based amperometric biosensor fabricated on a platinum-coated nanoporous alumina electrode has been described. This fabricating process introduced artificial porosity into the PPy film, and the template pore sizes were carefully chosen to match the size of the glucose oxidase (GOx) molecule. The PF−6 -doped PPy film was synthesized with 0.05 M pyrrole and 0.1 M NaPF6 at a current density of 0.3 mA/cm2 for 90 s. Immobilization was done by physically adsorbing 5 μL of GOx on the nanoporous PPy film. Glutaraldehyde (0.1 wt.%, 5 μL) was used for cross-linking. The synthesized films were characterized by using an electrochemical technique and scanning electron microscopy (SEM). Amperometric responses were measured as a function of different concentrations of glucose at 0.4 V. Nanoporous electrodes lead to high enzyme loading, whereas the use of a cross-linking agent increased stability, sensitivity, reproducibility, repeatability, and shelf life.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

57

Issue

8

Start Page

1621

End Page

1626

Number of Pages

6

ISSN

1557-9662

Location

United States

Publisher

IEEE

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Kyūshū Kōgyō Daigaku;

Era Eligible

Yes

Journal

IEEE transactions on instrumentation and measurement.

Exports

CQUniversity

Exports