Bromilow, L, Stanton, R, and Humphries, B. A structured e-investigation into the prevalence and acceptance of smartphone applications by exercise professionals. J Strength Cond Res XX(X): 000-000, 2020-The primary purpose of this study was to examine the prevalence and acceptance of smartphone applications by exercise professionals when interacting with clients and patients. A 29-item anonymous online survey was designed, containing separate sections on demographics, smartphone proficiency, benefits and barriers to using smartphones, and use of smartphones in a professional setting. Accredited members of the Australian Strength and Conditioning Association, and Exercise and Sports Science Australia received an information sheet through organizational communication channels, inviting them to participate. Two hundred forty-nine exercise professionals completed the survey, with men (71%; n = 176) accounting for most of the respondents. Proficiency using smartphone applications is predominantly-advanced (37%; n = 92), intermediate (33%; n = 82), or expert (14%; n = 35). Identified strategies to find smartphone applications included personal searches (67%; n = 167) and colleague recommendations (55%; n = 137). Reported benefits include fast access to information (67%; n = 167), saves time for record keeping (56%; n = 141), and allows performance tracking (55%; n = 138). Almost all respondents (92%; n = 229) identified barriers, such as inexperience with using particular applications (42%; n = 105). Almost all respondents (96%; n = 239) reported they would recommend smartphone applications to clients and patients, primarily for self-tracking (53%; n = 132). Smartphone use among exercise professionals is prevalent; however, application and sensor technology are reluctantly underused. Increasing acceptance requires embedding within educational curricula, recognition from professional organizations, and collaboration with, to maximize the potential capabilities of smartphone technology within working environments.