File(s) not publicly available

A protocol for a highly consistent, high level production in vivo of plasmodium falciparum oocysts and sporozoites

journal contribution
posted on 06.12.2017, 00:00 by M Looker, Andrew Taylor-RobinsonAndrew Taylor-Robinson
Investigation of the intimate relationship between the human malaria parasite Plasmodium falciparum and its Anopheles vector requires the reliable production and isolation of successive sexual stages of the parasite from infected mosquitoes. Such an advance in propagation would benefit a range of molecular, cellular, immunochemical and transmission-blocking research studies. Parasite cultivation, mosquito rearing, infection and subsequent dissection of mosquitoes are all highly technical procedures that require both skill and experience to perform with competence. Furthermore, to produce mosquitoes of an appropriate age to infect during the short period in which parasites are viable for infection demands precise planning in order to coordinate the interacting life cycles of the parasite and vector. Here, a protocol is described for the complete development of P. falciparum within Anopheles stephensi. A very consistent, high level production in vivo of P. falciparum oocysts and sporozoites is demonstrable by dissection of the mosquito midgut and salivary glands, respectively.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

5

Issue

13

Start Page

985

End Page

993

Number of Pages

9

eISSN

2156-8502

ISSN

2156-8456

Location

China

Publisher

Scientific Research

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Not affiliated to a Research Institute; School of Medical and Applied Sciences (2013- ); University of Leeds;

Era Eligible

Yes

Journal

Advances in bioscience and biotechnology.