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A novel experimental platform for the microscopic observation of germination and post-germination events in Phytopathogenic microfungi

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journal contribution
posted on 06.12.2017, 00:00 by Noel SammonNoel Sammon, Keith Estate Of HarrowerKeith Estate Of Harrower
This method describes the optimisation of compound microscopy for the observation of fungal spore germination, and post-germination events including appressorial formation, migration of nuclei, and development of the vacuolar-microtubular systems. Central to this method is the cellulosic microscope slide, developed as an extension to the method for observing fungal mycelial growth devised by Harrower (1985). The cellulosic microscope slide is a transparent experimental platform developed as a dry substrate speciffically to enable the observation of fungal spore germination under various desiccation and relative humidity regimes, but which also proved to be invaluable for the observation of post-germination events.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Volume

25

Issue

2

Start Page

61

End Page

64

Number of Pages

4

ISSN

1441-5526

Location

Narrabundah, ACT

Publisher

Australasian Mycological Society

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Primary Industries Research Centre; TBA Research Institute;

Era Eligible

Yes

Journal

Australasian mycologist.