Previous research has examined a range of demographic variables that have been shown to influence an individual’s attitude toward, and in turn their treatment of, animals. Little is known, however, about the effect of certain occupations upon these attitudes. The current study examines attitudes toward animals and the propensity for aggression within a sample of farmers and meatworkers in Queensland, Australia. Recent findings and publicity around the effects of employment (and cases of deliberate animal cruelty) within these industries indicates that this is an area in need of investigation from both human and animal welfare perspectives. The implications of the current findings for the meat-working industry and for the field of human-animal studies are discussed.