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A demonstration of the use of ultra-performance liquid chromatography–mass spectrometry [UPLC/MS] in the determination of amphetamine-type substances and ketamine for forensic and toxicological analysis

journal contribution
posted on 06.12.2017, 00:00 authored by L Apollonio, D Pianca, I Whittall, W Maher, Jennelle KydJennelle Kyd
We have recently seen the emergence of ultra-performance liquid chromatography (UPLC) coupled to mass spectrometry as an alternative to traditional high-performance liquid chromatography techniques. The strengths of UPLC technology promote the ability to separate and identify drug compounds with significant gains in resolution and sensitivity and marked reductions in the overall time of analysis. As increased throughput is the desire of the practical toxicology laboratory, the aim of this study was to trial commercially available technology by assessment of the separation of several commonly encountered amphetamine-type substances. From injection of a poly-drug reference standard and whole blood extract, we successfully separated and identified amphetamine, methamphetamine, ephedrine, pseudoephedrine, phentermine, MDA, MDMA, MDEA and ketamine in less than 3 min using the Acquity UPLC-Micromass Quattro Micro API MS instrumentation (Waters Corporation, USA). In addition to this significant reduction in overall run time, all peaks exhibited acceptable resolution using selected ion recording (SIR), with analysis indicating the capability to separate 5–11 peaks in 1.75 min using the current system parameters. From this introductory data, it is therefore indicated that the technological advancements defining ultra-performance liquid chromatography will allow it to serve as a powerful analytical tool for rapid throughput analysis.

History

Volume

836

Issue

1-2

Start Page

111

End Page

115

Number of Pages

5

ISSN

1570-0232

Location

Netherlands

Publisher

Elsevier BV

Language

en-aus

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Government Analytical Laboratory; University of Canberrra;

Era Eligible

Yes

Journal

Journal of chromatography B.