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A 28-day repeat dose toxicity study of steroidal glycoalkaloids, α-solanine and α-chaconine in the Syrian Golden hamster

journal contribution
posted on 05.02.2020, 00:00 by S Langkilde, T Mandimika, M Schrøder, O Meyer, W Slob, A Peijnenburg, M Poulsen
Glycoalkaloids α-solanine and α-chaconine are naturally present toxicants in the potato plant (Solanum tuberosum). Human intake of high doses of glycoalkaloids has led to acute intoxication, in severe cases coma and death. Previous studies have indicated that the ratio of α-solanine to α-chaconine may determine the degree and nature of the glycoalkaloid toxicity in potatoes, as the toxicity of the two alkaloids act synergistically. The aim of the present study was to investigate whether an altered ratio of α-solanine and α-chaconine would reduce the toxicity of the glycoalkaloids. The Syrian Golden hamster was given daily doses of α-solanine and α-chaconine by gavage for 28 days. Doses of up to 33.3 mg total glycoalkaloids/kg body weight were applied in ratios of 1:3.7 and 1:70 (α-solanine:α-chaconine). Administration of the highest doses of both ratios resulted in distended and fluid filled small intestines and stomach. Animals receiving the ratio with the reduced content of α-solanine were less affected compared to those receiving the other ratio. Gene expression profiling experiments were conducted using RNA from epithelial scrapings from the small intestines of the hamsters administered the highest doses of the glycoalkaloid treatments. In general, more differential gene expression was observed in the epithelial scrapings of the hamsters fed the ratio of 1:3.7. Mostly, pathways involved in lipid and energy metabolism were affected by the ratio of 1:3.7. © 2009 Elsevier Ltd. All rights reserved.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

47

Issue

6

Start Page

1099

End Page

1108

Number of Pages

10

ISSN

0278-6915

Publisher

Elsevier, UK

Peer Reviewed

Yes

Open Access

No

Acceptance Date

27/01/2009

External Author Affiliations

Technical University of Denmark; RIKILT-Institute of Food Safety, National Institute for Public Health and the Environment, The Netherlands

Era Eligible

Yes

Journal

Food and Chemical Toxicology

Exports