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Novel toxic activity associated with the cyanobacteria Limnothrix : use of screening assays for detection

conference contribution
posted on 2017-12-06, 00:00 authored by S Froscio, H Lau, C Bernard, Larelle FabbroLarelle Fabbro, A Humpage
Development of biochemical screening assays for cyanobacterial toxins allows identification of toxins based on known activity. While this may be diagnostic for the toxin or toxin class that the assay has been designed for (e.g. microcystins, cylindrospermopsins) there is also potential to pick up new, unidentified toxins. In this example, screening cyanobacterial samples for the toxin cylindrospermopsin led to the identification of novel toxic activity from the cyanobacterium Limnothrix.The cell-free protein synthesis assay was inhibited by the Limnothrix extract as would be expected when cylindrospermopsin is present. However analysis by immunoassay (ELISA) and analytical techniques (HPLC, LC-MS) could not detect cylindrospermopsin itself. PCR amplification of genes associated with cylindrospermopsin production was also absent. Further characterisation revealed that the toxic responses induced by the Limnothrix extract could not be attributed to other known cyanobacterial toxins. Distinctive toxic effects of the Limnothrix extract in mammalian cells include significant ATP depletion and markedgranulation of cells as observed by both microscopy and flow cytometry. Current work aimsto identify and characterise the toxic agent present. This case highlights how biochemical screening assays can complement analytical techniques for the identification of toxic activity in cyanobacterial samples. Adequate validation of such techniques is required.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Start Page

48

Start Date

2010-01-01

Location

Melbourne, Vic.

Publisher

Water Quality Research Australia (WQRA)

Peer Reviewed

  • No

Open Access

  • No

External Author Affiliations

Australian Water Quality Centre; Centre for Environmental Management; Institute for Resource Industries and Sustainability (IRIS);

Era Eligible

  • No

Name of Conference

National Cyanobacterial Workshop