This paper presents different techniques used in fabricating polypyrrole substrates for the use in disposable blood glucose measurement sensors. It compares two main fabrication methods of templated electrodepositing and pulse depositing of polypyrrole. Paper compares the two types of sensors using their sensitivities and linear ranges with respect to production cost. The results show that polypyrrole is a very good substrate material which can be easily fabricated into a nano-surface to adsorb higher amount of glucose oxidase.