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Enhanced adsorption of glucose oxidase by introducing artificial porosity into polypyrrole based glucose biosensors

conference contribution
posted on 06.12.2017, 00:00 by E Mala Ekanayake, Daluwathu Preethichandra, K Kaneto
A polypyrrole (PPy) based biosensor fabricated on a Pt plated nano-porous alumina electrode has been described. By selecting nano-porous electrodes, the enzyme adsorption has been increased and the usage of a cross linking agent has enhanced the performances of the sensor remarkably. A thin film of PPy/PF6 was synthesized with 0.05M Pyrrole, 0.1M NaPF6 at a current density of 0.3mA/cm2 for 90s. The immobilization was done by physically adsorbing 5µL of Glucose Oxidase (GOx) on PPy thin film. Glutaraldehyde (0.1wt.%, 5µL) was used for cross-linking. The synthesized films were characterized by using amperometric electrochemical technique and scanning electron microscopy (SEM). Amperometric responses were measured as a function of different concentrations of glucose. Nanoporous electrodes lead to high enzyme loading. Stability, sensitivity, reproducibility and repeatability have been increased with the usage of cross-linking.

History

Start Page

1

End Page

4

Number of Pages

4

Start Date

01/01/2007

ISBN-10

1424405882

Location

Warsaw, Poland

Publisher

IEEE

Place of Publication

Piscataway, NJ

Peer Reviewed

Yes

Open Access

No

External Author Affiliations

Kyūshū Kōgyō Daigaku;

Era Eligible

Yes

Name of Conference

IEEE Instrumentation and Measurement Technology Conference

Exports

CQUniversity

Exports