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Mosquitoes and parasites. I, Rearing of Anopheles stephensi mosquitoes. III, Procedures required to generate Anopheles stephensi mosquitoes infected with the human malaria parasite, Plasmodium falciparum. IV:D, Production of Plasmodium falciparum oocysts and sporozoites
chapterposted on 06.12.2017, 00:00 by M Looker, Andrew Taylor-Robinson
The production of Anopheles stephensi mosquitoes infected with the human malaria parasite, Plasmodium falciparum, involves the propagation of parasite and vector life cycles in parallel. It is a technically difficult procedure which requires considerable pre-planning and attention to detail in order to be successful. In particular, the precise timing of each stage of the process is of paramount importance to ensure a consistant supply of infected mosquitoes for the purposes of research. This 'timeline' is a valuble organisational tool in that it identifies, in short-hand form, precisely what tasks need to be performed on a day-to-day basis. In our laboratory, we have found this schematic representation to be useful not only as an aide memoire for the experienced parasitologist but also as a teaching aid to researchers new to malaria.