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Cultivation of Plasmodium falciparum gametocytes for mosquito infectivity studies

chapter
posted on 06.12.2017, 00:00 authored by M Looker, Andrew Taylor-RobinsonAndrew Taylor-Robinson
Gametocytes of P. falciparum are established from continuously maintained cultures of asexual blood stage parasites. Gametocytes begin to form in significant numbers in blood culture only following daily dilution with fresh RBC and medium for several days. In order for gametocytes to comprise a substantial proportion of PRBC, it is necessary for asexual PBRC to reach a high density and become stressed in vitro before conversion to gametocyte production. This occurs typically 6 to 8 d after the dilution of a culture.Gametocytes then require at least a further 8 d to reach maturity. Cultures for gametocyte production are therefore maintained for a minimum of 14 d without further dilution with fresh RBC in order to obtain a large proportion of mature and infectious gametocytes.We routinely perform gametocyte feeds to mosquitoes using cultures propagated continuously for the preceding 14 to 17 d.

Funding

Category 1 - Australian Competitive Grants (this includes ARC, NHMRC)

History

Start Page

138

End Page

141

Number of Pages

4

ISBN-10

0930009649

Publisher

EVIMalaR

Place of Publication

Glasgow, UK

Open Access

No

External Author Affiliations

School of Medical and Applied Sciences (2013- ); TBA Research Institute; University of Leeds;

Era Eligible

No

Edition

6th